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Addgene inc vsvg
Vsvg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 14643 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmd2%2Eg+(vsvg)+plasmid/pMD2%2EG+(Plasmid+%2312259)/pm41916275-716-32-34
Average 98 stars, based on 14643 article reviews
vsvg - by Bioz Stars, 2026-09
98/100 stars

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Related Articles

Plasmid Preparation:

Article Title: Pre-existing cell states predict resistance to multiple treatments.
Article Snippet: .. Per plate, we combined 500 μL of OPTI-MEM (Gibco, 31985062) with 80 μL of PEI (Polysciences, 23966-1) in a tube while combining 500 μL OPTI-MEM with 10 μg barcode plasmid, 5 μg VSVG (pMD2.G, Addgene #12259), and 7.5 μg psPAX2 (Addgene #12260) in a separate tube. ..

Article Title: Reprogramming mRNA localization by targeted RNA-protein interference
Article Snippet: .. Briefly, lentivectors and packaging plasmids pMD2.VSVG (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260) were transfected with PolyJet DNA transfection reagent (SignaGen; SL100688). ..

Article Title: MPI-Guided Photothermal Therapy of Prostate Cancer Using Stem Cell Delivery of Magnetotheranostic Nanoflowers
Article Snippet: .. Nanoluc luciferase reporter lentiviral vectors were produced by co-transfecting a CMV promotor-driven Nanoluc vector (pNL1.1.CMV[Nluc/CMV], cat# N1091, Promega,), with VSVG envelope glycoprotein pMD2.G (plasmid #12259) and psPAX2 (plasmid#12260) (Addgene) in 293FT cells. ..

Article Title: Dasatinib resensitizes BRAF/MEK inhibitor efficacy in patient-derived xenografts from patients with progression on BRAF/MEK inhibitor treatment
Article Snippet: psPAX2 packaging plasmid , Addgene , Cat# 12260. .. pMD2.G (VSVG envelope plasmid) , Addgene , Cat# 12259. .. Lentiviral barcode plasmid (FateMap vector) , Goyal et al., 2023 , –.

Transfection:

Article Title: Reprogramming mRNA localization by targeted RNA-protein interference
Article Snippet: .. Briefly, lentivectors and packaging plasmids pMD2.VSVG (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260) were transfected with PolyJet DNA transfection reagent (SignaGen; SL100688). ..

Article Title: The 9p21.3 Coronary Artery Disease Risk Locus Modulates Vascular Cell-State Transitions via Enhancer-Driven Regulation of MTAP
Article Snippet: After 48 hours. fluorescence was measured on a CytoFLEX S. The ratio of GFP/RFP in dCas9-KRAB cells relative to the parental line was calculated using FlowJo software and used to assess effective repression. .. We generated Lentiviral cells by transfecting HEK293T cells at about 60% confluency with 1,250 ng sgRNA, and second generation lentiviral packaging vectors 1250 ng psPAX2 (Addgene #12259) and 250 ng of VSVG (Addgene#12259) using TransIT-LT1 transfection reagent. ..

Generated:

Article Title: The 9p21.3 Coronary Artery Disease Risk Locus Modulates Vascular Cell-State Transitions via Enhancer-Driven Regulation of MTAP
Article Snippet: After 48 hours. fluorescence was measured on a CytoFLEX S. The ratio of GFP/RFP in dCas9-KRAB cells relative to the parental line was calculated using FlowJo software and used to assess effective repression. .. We generated Lentiviral cells by transfecting HEK293T cells at about 60% confluency with 1,250 ng sgRNA, and second generation lentiviral packaging vectors 1250 ng psPAX2 (Addgene #12259) and 250 ng of VSVG (Addgene#12259) using TransIT-LT1 transfection reagent. ..

Luciferase:

Article Title: MPI-Guided Photothermal Therapy of Prostate Cancer Using Stem Cell Delivery of Magnetotheranostic Nanoflowers
Article Snippet: .. Nanoluc luciferase reporter lentiviral vectors were produced by co-transfecting a CMV promotor-driven Nanoluc vector (pNL1.1.CMV[Nluc/CMV], cat# N1091, Promega,), with VSVG envelope glycoprotein pMD2.G (plasmid #12259) and psPAX2 (plasmid#12260) (Addgene) in 293FT cells. ..

Produced:

Article Title: MPI-Guided Photothermal Therapy of Prostate Cancer Using Stem Cell Delivery of Magnetotheranostic Nanoflowers
Article Snippet: .. Nanoluc luciferase reporter lentiviral vectors were produced by co-transfecting a CMV promotor-driven Nanoluc vector (pNL1.1.CMV[Nluc/CMV], cat# N1091, Promega,), with VSVG envelope glycoprotein pMD2.G (plasmid #12259) and psPAX2 (plasmid#12260) (Addgene) in 293FT cells. ..



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